Depletion of the THOC5 gene did not cause pathological alteration to liver or kidney. (A) Rosa26-CreERT2:THOC5 (flox/flox) mice were treated with (+) or without (−) tamoxifen and six days after the first tamoxifen injection, kidney and liver were fixed in formalin. Paraffin sections were supplied for immunohistochemical staining using cleaved caspase 3 antibody. Bars represent 100 μm. (B) Protein extracts from liver obtained from the same mice as in (A) were supplied for THOC5, cleaved (cl.) caspase 3, caspase 3 and actin specific immunoblot. (C) Nuclear (Nuc) or cytoplasmic (Cyt) RNA from 20 μg of liver tissues before or after the tamoxifen treatment were supplied for the THOC5 specific RT-PCR or nuclear (Nuc) or cytoplasmic (Cyt) protein extracts from same tissues (200 μg of liver tissue) were supplied for GAPDH and Histone H3 specific immunoblot (Blot). (D) Aliquots of the same RNAs in (C) were supplied for Albumin, Transferrin and beta actin specific semi-quantitative RT-PCR. Cycles: Number of amplification cycles.
Saran et al. BMC Cell Biology 2013 14:51 doi:10.1186/1471-2121-14-51