Co-immunoprecipitation and Western blot analysis. (A) The upper panel shows the presence of caldendrin in IP complex of MAST antisera on probing with antisera raised to caldendrin. Signals of the same molecular weight are also present in the lysates (without immunoprecipitation) from mouse testis, kidney and brain indicating the presence of caldendrin in these tissues as well. In the lower panel IP with caldendrin antisera that is probed with MAST antisera shows the presence of MAST protein in the IP complex and testis lysate only. MAST is not present in other tissues such as kidney, spleen and brain. (B) Co-immunoprecipitation of MAST and calreticulin: Antisera of MAST used for IP and probed with calreticulin antibody shows the presence of calreticulin protein in the IP complex and the lysates from testis, kidney spleen and brain, with high expression in kidney. Calreticulin antibody used for immunoprecipitation and probed with antisera of MAST protein shows the presence of MAST protein in the IP complex and testis lysate only.
Bhattacharya et al. BMC Cell Biology 2013 14:50 doi:10.1186/1471-2121-14-50