Additional file 1: Figure S1.

Expression of markers related to three germ layer within EBs. Semiquantitative RT-PCR measurement of markers related to the three germ layer (endoderm: a-Fetoprotein, GATA-6; mesoderm: Brachyury; ectoderm: TuJ1, Map2) within 3d- and 5d-EBs derived from cells subject to integrin disruption and controls. Experiments were performed in triplicate, and the transcripts for GAPDH were used for internal normalization.

Format: TIFF Size: 88KB Download file

Zeng et al. BMC Cell Biology 2013 14:5   doi:10.1186/1471-2121-14-5