Table 2

ADF and cofilin suppression affects MTLn3 cell polarization after EGF stimulation
EGF stimulation Subcategory Control ADF KD Cofilin KD
0 sec Polarized Crescent-shaped 68.3 ± 1.6 33.6 ± 1.2*** 13.1 ± 0.6***
Kite-shaped 31.7 ± 1.6 66.4 ± 1.2*** 86.4 ± 0.6***
Non-polarized Apolar 91.3 ± 0.3 91.0 ± 0.1 71.5 ± 1.3
Bipolar 8.7 ± 0.3 9.0 ± 0.1 9.7 ± 1.2
Multipolar 0.0 0.0 18.8 ± 1.5
60 sec Polarized Crescent-shaped 75.0 ± 1.1 75.2 ± 1.9 49.3 ± 0.7***
Kite-shaped 25.0 ± 1.1 24.8 ± 1.9 50.7 ± 0.7***
Non-polarized Apolar 91.4 ± 0.3 66.6 ± 0.2*** 100.0***
Bipolar 8.6 ± 0.3 34.4 ± 0.2*** 0.0
Multipolar 0.0 0.0 0.0
180 sec Polarized Crescent-shaped 79.8 ± 0.2 80.6 ± 0.4 48.5 ± 0.5***
Kite-shaped 20.2 ± 0.2 19.4 ± 0.4 51.5 ± 0.5***
Non-polarized Apolar 91.2 ± 0.3 95.7 ± 0.3*** 100.0***
Bipolar 8.8 ± 0.3 4.3 ± 0.3*** 0.0
Multipolar 0.0 0.0 0.0

Polarized and non-polarized cells were subcategorized as in Figure 4A and the percentage of cells in each category was scored. Data are expressed as mean ± SEM, n ≥ 250 cells in each experiment, three independent experiments. ***p < 0.001 versus control.

Tahtamouni et al.

Tahtamouni et al. BMC Cell Biology 2013 14:45   doi:10.1186/1471-2121-14-45

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