shRNA knockdown of β-catenin is associated with the regulation of Wnt pathway, EMT, and pluripotency networks. A. The top panel shows that HONE1 hybrid cells have an up-regulated expression of β-catenin by qPCR analyses, compared to the parental HONE1 cells. MCHs 4.5 and 4.6 are infected by scramble (empty bars) and β-catenin shRNAs (black bars). The number in parenthesis refers to the growth passages of cells after infection. Fold-changes reflect inhibitory expression of β-catenin in these cells compared to corresponding scramble negative controls. β-catenin expression is reduced in all four cell lines, as shown in the bottom panel. B. qPCR analyses show inhibited expression of Nanog, Oct4, Sox2, and Klf4 in β-catenin shRNA infected cells. C. Western blot assay confirms that β-catenin shRNAs inhibit expression of β-catenin, E-cadherin, core stem cell factors (Oct4, Klf4, and Sox2), and EMT factors (Zeb1 and Fn1) in the treated HONE1 hybrid cells.
Cheng et al. BMC Cell Biology 2013 14:44 doi:10.1186/1471-2121-14-44