Table 2

Summary of immunohistochemical staining of FTSEC spheroids
FTE02 FTE03 FTE283 FTE284 TISSUE
Marker 2D 3D 2D 3D 2D 3D 2D 3D FTE03 FTE04
AE1:AE3 ++ +/− +++ + +++ ++++ ++++ ++ ++++ ++++
VIMENTIN +++ ++ ++++ +++ ++++ +++ ++++ +++ ++++ +++
LAMININ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++ ++
MUC-1 ++ +/− + - + +++ ++ - ++++ +++
CA125 + - + - +/− ++ ++ - +++ +++
PAX8 + - ++ +/− +++ +++ ++ +/− +++ ++
TP53 (Do7) +++ +/− +++ + ++ + +++ +/− + +/−
MIB-1 (%) 70% <5% 70% <5% 70% <5% 50% <5% <5% <5%

Immunohistochemistry was performed on five FTSEC cultures grown as 2D monolayers and as 3D spheroids, and compared to two fresh tissue specimens. Staining of pan-cytokeratin (AE1:AE3), mucin-1, CA125, PAX8 and E-Cadherin showed no significant changes in 2D versus 3D cultured cells. Laminin showed high levels of expression in all samples and expression along the basement membrane of fallopian tube epithelial cells in vivo. TP53 and MIB1 expression were high in 2D cultured cells, but low in 3D cultured cells and in vivo.

Lawrenson et al.

Lawrenson et al. BMC Cell Biology 2013 14:43   doi:10.1186/1471-2121-14-43

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