Figure 6.

Substitution of Ser846 prevents effects of PP6c knockdown on E-cadherin localization. Either myc tagged mouse E-cadherin wild type (WT) or S846A were inserted into the vector with non-coding (Con.) or PP6c shRNA for stable expression in Caco-2 cells by lentiviral infection. Co-expression of myc-E-cadherin and shRNA were induced after cell confluence. (A) Immunofluorescent staining of myc-E-cadherin. (B) Quantification of fluorescence intensity of myc-E-Cadherin in (A) by line scans (10 μm). (C) The full width at half maximum (FWHM) of each line scan was calculated and the average of 38 scans [26] are presented (mean + SE).

Ohama et al. BMC Cell Biology 2013 14:42   doi:10.1186/1471-2121-14-42
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