Acute exposure of mature human adipocytes to ATRA does not induce UCP1 expression. ATRA (1 μM) was supplemented to mature SGBS adipocytes (A), hMADS adipocytes (B) and primary human white preadipocytes (HPrAD) (C) from day 12 and harvested after 24 h. (D) Protein levels of UCP1 with GAPDH used as a loading control in SGBS and hMADS adipocytes. (A-C) Data represents mean + SEM (n = 3). *, p < 0.05 versus vehicle-treated cells.
Murholm et al. BMC Cell Biology 2013 14:41 doi:10.1186/1471-2121-14-41