RAR subtype-selective ligands induce UCP1 expression in WT MEFs. Total RNA was harvested at day 8 (panel A) or at the indicated days (panel B) and analyzed by RT-qPCR. Relative mRNA expression levels of FABP4, UCP1 and RARβ were determined by normalization to TBP. (A) RAR subtype-selective ligands AM580, tazarotene and CD1530 were supplemented from day −2 to day 8 at the concentrations indicated. (B) RAR subtype-selective ligands AM580 (1 μM), tazarotene (1 μM) and CD1530 (1 μM) were acutely supplemented to mature adipocytes from day 8 and harvested after 24 h. Data represents mean + SEM (n = 3). *, p < 0.05 versus vehicle-treated cells.
Murholm et al. BMC Cell Biology 2013 14:41 doi:10.1186/1471-2121-14-41