TTNPB mimics and BMS493 inhibits the effects of ATRA in WT MEFs. Total RNA was harvested at the indicated days (day 8 in panels A and C) and analyzed by RT-qPCR. Relative mRNA expression levels of FABP4, UCP1 and RARβ were determined by normalization to TBP. (A) TTNPB was supplemented to differentiating cells from day −2 to day 8 at the concentrations indicated. (B) TTNPB (1 nM) was acutely supplemented to mature adipocytes (day 8) and harvested after 24 h. (C) Treatment with BMS493 (1 μM) and/or ATRA (1 μM) from day −2 to day 8. (D) Acute supplementation of BMS493 (1 μM) and/or ATRA (1 μM) to mature adipocytes from day 8 and harvested after 24 h. Data represents mean + SEM (n = 3). *, p < 0.05 versus vehicle-treated cells. ▲, p < 0.05 versus ATRA-treated cells.
Murholm et al. BMC Cell Biology 2013 14:41 doi:10.1186/1471-2121-14-41