Additional file 6.

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Vanadate antagonizes the inhibitory effect of Src kinase inhibitor on membrane ruffling. Ric10 cells were cultured in pmDM for 24 h and sequentially observed under phase contrast microscopy by time-lapse recording.Images were recorded every 2.5 min by phase-contrast time-lapse microscopy. Membrane ruffling in pmDM (Additional file 6) was suppressed in pmDM supplemented with SU6656 (Additional file 7). Membrane ruffling in pmDM supplemented with vanadate (Additional file 8) was not suppressed in pmDM supplemented with SU6656 and vanadate (Additional file 9).

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Mukai and Hashimoto BMC Cell Biology 2013 14:37   doi:10.1186/1471-2121-14-37