BMI significantly affected ASC early and late osteogenesis potential in vitro. ASC osteogenesis was induced using a cocktail medium for 16 days. Calcium deposition was assessed by Alizarin red staining (ARS) at a. day 8, and b. day 16. c. Representative micrographs of Alizarin Red Staining (ARS) in BMI-ASCs at day 15. d, e. Regression analyses of differentiation data that was analyzed at days 8 and 16 using least squares ordinary fit. R2 value reflected a strong correlation at both early and late osteogenesis time points. Values reported as N ± SE; * p > 0.05, ** p > 0.001; *** p > 0.0001.
Frazier et al. BMC Cell Biology 2013 14:34 doi:10.1186/1471-2121-14-34