Caspase-7 and-3 have distinct effects on ROS production during apoptosis. (A) Expression levels of effector caspases in Casp KO MEFs were determined by western blot. (B) WT, Casp7-/-, Casp3-/- and Casp3-/-7-/- MEFs were withdrawn from serum for 12 hr. ROS production was determined by flow cytometry. Treatment with 50 μg/ml antimycin A for 30 min was used as a positive control. Data are representative of at least 3 independent experiments.
Brentnall et al. BMC Cell Biology 2013 14:32 doi:10.1186/1471-2121-14-32