Additional file 5: Figure S5.
Chronic GL conditions reduce insulin synthesis and vesicle transport. Rat islets or NIT1 cells were cultured under normal conditions (control) or with 16.7 mM glucose and 500 μM palmitate for 72 h (GL). Post 72 h, RNA was isolated from both islets and cells. The mRNA levels of PDX1 (A) and Insulin (B) were measured in islets and Rab27a levels (C) in cells were measured as described in the materials and methods. Data are expressed as mean±SEM and statistical analysis was performed using the unpaired Student’s t-test. (*P<0.05, **P<0.01 and ***P<0.001, n=4).
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Somesh et al. BMC Cell Biology 2013 14:31 doi:10.1186/1471-2121-14-31