Additional file 2: Figure S2.

Chronic glucolipotoxic conditions increase endoplasmic reticulum stress and apoptosis in pancreatic β-cells. NIT1 cells were cultured under normal conditions (control) or with 16.7 mM glucose and 500 μM palmitate for 72 h (GL). Post 72 h, changes in ER stress markers (A) were analyzed using anti-CHOP, anti-BiP and phospho-specific EIF2a antibodies; β-actin was used as the internal control. Both Caspase-3 activity (B) and cell viability (MTT assay) were measured in NIT1 cells as described in the Additional file 6. Data are expressed as mean±SEM and statistical analysis was performed using the unpaired Student’s t-test. (*P<0.05, **P<0.01 and ***P<0.001, n=4).

Format: PDF Size: 30KB Download file

This file can be viewed with: Adobe Acrobat Reader

Somesh et al. BMC Cell Biology 2013 14:31   doi:10.1186/1471-2121-14-31