Analysis of the level of telomere clustering in pools of heterologous chromosomes. Distribution of the number of telomeric spots (pp, qq, pq) per nucleus in neutrophils (resting in green, LPS-activated in red) using a pool of p, q and p + q telomeric probes labelling the telomeres of: (a) the 5 submetacentric chromosomes (SSC1 to SSC5), (b) the 2 subtelocentric chromosomes (SSC6 and SSC7), (c) the 5 metacentric chromosomes (SSC8 to SSC12), (d) the 6 telocentric chromosomes (SSC13 to SSC18). The x-axis displays the number of telomeric spots counted and the y-axis the % of nuclei harbouring the numbers of spots concerned. The dashed lines indicate the expected number of spots according to the number of telomeres labelled in the pool. The P values show comparisons between the two states (resting and activated) using a χ2 test.
Mompart et al. BMC Cell Biology 2013 14:30 doi:10.1186/1471-2121-14-30