Figure 6.

Sub-cellular localization of Rheb on Golgi. A) HEK293 cells; B) HeLa cells were transiently transfected with EGFP-Rheb. Twenty-four hours after transfection cells were stained with 5 μM BODIPY TR C5 ceramide and live cells analyzed by confocal microscopy. The images reveal that Rheb localizes to the Golgi. Bar 8 μm.