Figure 5.

Sub-cellular localization of Rheb on ER. A) HEK293 cells; B) HeLa cells were transiently transfected with EGFP-Rheb. Twenty-four hours following transfection cells were stained with 1 μM ER Tracker red and live cells analyzed by confocal microscopy. The images reveal that Rheb localizes to the ER Bar 8 μm.