Effect of syndecan-2 blockade on actin filament formation in HCT-116 cells. HCT-116 cells, previously grown on top of fibroblast ECM for three days, were incubated with anti-syndecan-2 antibody (Anti-Syn-2), IgG (IgG) or no antibodies (Ctrl) and then plated on stromal fibroblast ECM-coated glass coverslips for different lengths of time. Actin filaments were stained. The image above was obtained by fluorescence microscopy. F-actin is labeled with phalloidin in green; nucleus is labeled with DAPI in blue. Scale mark: 20 μm.
Vicente et al. BMC Cell Biology 2013 14:25 doi:10.1186/1471-2121-14-25