Effect of stromal fibroblast ECM on the synthesis of GAGs by Caco-2 and HCT-116 cells. Cancer cells were cultured in the absence (Ctrl) or presence (Fibrob. ECM) of stromal fibroblast ECM. GAGs were labeled with [35S]Na2SO4 and were purified from the culture medium (MEDIUM), cancer cells (CELL) and the matrix (MATRIX) produced by Caco-2 or HCT-116 cells. (A) The content of GAGs from these compartments was analyzed by agarose gel electrophoresis in 1,3-diaminepropane acetate buffer (0.05-M pH 9.0). The gel was exposed to a screen and the bands were identified using an image analysis system, the Cyclone® Storage Phosphor System-Packard Instrument. (B) Quantification was performed by densitometry with Opti-Quanti Software. Heparan sulfate (HS), chondroitin sulfate (CS). *p ≤ 0.05 compared to control.
Vicente et al. BMC Cell Biology 2013 14:25 doi:10.1186/1471-2121-14-25