Table 1

Effects of high glucose and NOS inhibitor L-NAME on the viability, NO release, eNOS expression and F-actin fluorescence intensity of human umbilical vein endothelial cells incubated in different media (as described in Figure2) for seven days
Group Cell viability (Optical density) NO concentration (μmol/L) eNOS expression (%) F-actin florescence intensity
Control 0.80±0.093 78.6±4.00# 73.7±4.40# 1083±261.7#
HG 0.64±0.061 47.0±10.56* 50.5±0.72* 1538±250.7*
FG 0.73±0.114 42.4±8.59* 39.6±0.37*# 1566±309.3*
HM 0.50±0.106* 58.7±13.34# 57.0±1.38*# 1057±316.1#
FM 0.74±0.098 57.1±11.24# 56.2±1.19*# 1110±385.5#
0.1mM 0.65±0.104 50.3±9.24* 48.5±1.48* 1501±228.9*
0.5mM 0.70±0.075 40.9±4.00* 40.4±0.82*# 1625±344.8*
1mM 0.59±0.156 35.4±0.69* 28.3±0.29*# 1785±323.4*#

Data shown in the table are means±SD. *, P<0.05, compared to the control group. #, P <0.05, compared to the constant high glucose group.

Chen et al.

Chen et al. BMC Cell Biology 2013 14:22   doi:10.1186/1471-2121-14-22

Open Data