Effects of high glucose and the NOS inhibitor L-NAME on the release of NO in human umbilical vein endothelial cells. NO concentrations in the culture supernatant were measured seven days after incubation of endothelial cells in various media. Control, continuous low glucose (5mM) media; HG, continuous high-glucose (25mM) media; FG, fluctuating glucose (25/5mM, alternated every 24h) media; HM, continuous low glucose (5mM) media containing 20mM mannitol; FM, low glucose (5mM) media with 0/20mM mannitol (alternated every 24h); 0.1mM, 0.5mM and 1mM, continuous low glucose (5mM) media containing 0.1, 0.5 or 1mM L-NAME, respectively. Data shown in the figure are mean ± SD. “*”, P<0.05, compared to the control group. “#”, P<0.05, compared to the constant high glucose group.
Chen et al. BMC Cell Biology 2013 14:22 doi:10.1186/1471-2121-14-22