Quantitative PCR (qPCR) analyses of viral replicative cDNA during early HIV-1 infection. MT2 cells were either mock-infected or infected with HIV-1 and then lysed for qPCR analyses at 0, 2, 4 or 6 hrs post-infection. HIV-1 cDNA synthesis was quantified by qPCR using primers to detect early (strong stop) or intermediate (gag) reverse-transcription products. HIV-1 cDNA copies were normalized to cell numbers using CCR5 DNA.
Jones et al. BMC Cell Biology 2013 14:21 doi:10.1186/1471-2121-14-21