The inflammatory cytokine profiles of LPS-stimulated macrophages. The ASCs and thioglycolate-elicited macrophages were transwell co-cultured and incubated for another 24 hours in the presence of 30 ng/ml LPS. The macrophage cells were collected, and total RNA was extracted for real-time PCR quantification of inflammatory cytokines IL-1α, IL-1β, TNFα, IL-6 and IL-10. The macrophage (MФ) alone and LPS stimulated macrophage (MФ+LPS) were used as controls. ## means P< 0.01 MФ+LPS vs MФ+LPS (WtASCs) (t-test, n=3); **means P< 0.01 MФ+LPS vs MФ+LPS (TwiASCs) (t-test, n=3); # means P< 0.05 MФ+LPS vs MФ+LPS (WtASCs) (t-test, n=3); and ¥ means P< 0.05 MФ+LPS(WtASCs) vs MФ+LPS (TwiASCs) (t-test, n=3).
Zhang et al. BMC Cell Biology 2013 14:20 doi:10.1186/1471-2121-14-20