Colony forming unit assays for TwiASCs and WtASCs. 100 cells were plated on 56.7 cm2 Nunc cell culture plates and incubated for 14 days. Cells were stained with 3% crystal violet, and colonies 2 mm or larger in diameter were counted. # means P< 0.05 vs WtASCs (t-test, n=5).
Zhang et al. BMC Cell Biology 2013 14:20 doi:10.1186/1471-2121-14-20