The expression of TAGLN is upregulated in gastric cancer-associated fibroblasts. A: characterization of gastric fibroblasts (ICC). a1: vimentin, positive. a2: α-SMA, positive. a3: fibronectin, positive. a4: cytokeratin, negative. Original magnifications: ×200. Scale bars, 100 μm. B: QRT-PCR analysis of TAGLN in CAF, CPF and NF. *, P < 0.05, **, P < 0.01, compared to NF. C: Western blot analysis of TAGLN in CAF, CPF and NF, TAGLN expression level was decreased successively. D: fibroblasts growth curve were determined by CCK-8 assay, CAFs grew quickier than the others. E: CAF had higher ability to enhance MKN-45’s migration ability than CPF and NF, ***, P < 0.001 compared to MKN-45. F: CAF had higher ability to enhance MKN-45’s invasion ability than CPF and NF, *, P < 0.05, **, P < 0.01, compared to MKN-45.
Yu et al. BMC Cell Biology 2013 14:17 doi:10.1186/1471-2121-14-17