ClustalW2 alignment of PPP1R2 and PPP1R2P3. Protein sequences for PPP1R2 and PPP1R2P3 were obtained from Uniprot database. Sequences were submitted to a ClustalW2 alignment. Relevant motifs/regions for PPP1R2/PPP1R2P3 binding to PPP1C are shown in open boxes. Important phosphorylation sites are indicated with black arrows above the residues and with the respective known kinase. * represent high conservation, : and . represent low conservation in which the substituted residue has respectively more and less similar properties. Black horizontal bars below the protein sequences show the coverage of the peptides obtained by mass spectrometry. Black horizontal dash bar indicates the peptide that allowed for the distinction between PPP1R2P3 from PPP1R2. Black horizontal square dot bar indicates the peptide that allowed distinguishing PPP1R2.
Korrodi-Gregório et al. BMC Cell Biology 2013 14:15 doi:10.1186/1471-2121-14-15