MMP9, EGFR, Snail, and KGF transcript levels in control and nimotuzumab-treated cells. mRNA levels of MMP9, EGFR, Snail, and KGF were measured by quantitative real-time RT-PCR, normalized against GAPDH, and the indicated% induction or reduction was compared with those in cells cocultured with nimotuzumab. Although, the level of EGFR remained same, MMP9 and Snail expression in Tca8113 and ACC cells was inhibited by nimotuzumab. Error bars indicate SDs, n=3. *, P<0.05.
Huang et al. BMC Cell Biology 2013 14:13 doi:10.1186/1471-2121-14-13