Effect of nimotuzumab on the cell cycle distribution of Tca8113 and ACC cells. After treated with medium or medium containing nimotuzumab for 90 min followed by treatment with hEGF (40 ng/ml) for 15 min, cell nuclei were fixed, stained with PI, and analyzed by flow cytometry. Cells were subdivided into two groups: hEGF group and hEGF+ nimotuzumab group. A: Representative histograms are shown that combination treatment resulted in a 9.9% and 5% accumulation of ACC-M and Tca8113 cells in G1 phase. B: The statistical results of cell cycle distribution of Tca8113 and ACC cells are shown.
Huang et al. BMC Cell Biology 2013 14:13 doi:10.1186/1471-2121-14-13