Nimotuzumab inhibits the Tca8113 and ACC cell proliferation. A: After treated with different concentration antibody in Tca8113 and ACC cells the growth curve was drawn through CCK-8 assay. The anti-proliferative effect peaked at 100, 200, and 100 μg/ml in ACC-2, ACC-M, and Tca8113 cells, respectively. B: The anti-proliferation was also evaluated by by Ki-67 immunostaining. Red fluorescence was reduced in all three cell lines, specifically in Tca8113 and ACC-M cells. Magnification, ×200. C: Quantitative analysis of positive Ki-67 Tca8113 and ACCs cells in different groups.
Huang et al. BMC Cell Biology 2013 14:13 doi:10.1186/1471-2121-14-13