Additional file 1: Figure S1.
JNK activity does not affect JIP1 binding to kinesin-1. (A). Co-precipitation of GFP-JIP1 mutants and endogenous JNK in Neuro2a cells. The same samples as shown in Fig. 2A were analyzed by WB with the indicated antibodies. (B). Neuro2a cells transiently expressing TAP-JIP1 were treated with SP600125 (20 μM) or DMSO (vehicle) for 2 hours. Lysates were precipitated with SA. SA, proteins bound to streptavidin sepharose. (C). RFP-JIP1-Neuro2a cells were transfected with expression vectors for T7-MEKK1 or pUC8 (empty vector). Lysates were immunoprecipitated with anti-RFP antibody and analyzed by WB with the indicated antibodies. *: non-specific band.
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Satake et al. BMC Cell Biology 2013 14:12 doi:10.1186/1471-2121-14-12