In vitro and in vivo differentiation of iPSCs generated with 4TF plus DNp73. In vitro differentiation through embryoid bodies, subsequently differentiate to mesoderm (a. bracchyury staining, b. DAPI staining, c. merged), to endoderm (d. PDX1, e. DAPI, f. merged), and ectoderm (g. Tuj1 staining, h. DAPI, and i. merged). Histological sections from teratomas generated in nude mice from iPSCs consist of tissues from all three germ layers stained with hematoxylin and eosin (HE). Magnification folds 400×, J. mesoderm (1. cartilage); k. endoderm (2. goblet cells); l. ectoderm (3. neural epithelium); m. non-differentiation cells.
Lin et al. BMC Cell Biology 2012 13:9 doi:10.1186/1471-2121-13-9