Additional DNp73 transduction increase human iPSC generation efficiency and kinetics at day 21. Timeline for human iPSC induction combined dnp73 withtraditional 4TF. Treatment with additional factor dnp73: culture plate had colony growth at d21 with Nanog expression and at d30 with the traditional iPS cells. a. Culture derived from 4 TF only, had only a few colonies stained by ALP+. b. While, in the culture derived from transduction of 4TF plus DNp73, many colonies appeared when stained with ALP positive at day 21. c. At day 30, large colonies seen with Nanog expression, with approx. 20 fold increase by comparison between 4TF only and 4TF plus DNp73. d. significant enhancement of gene expression oct4 (/) and Nanog (/) by Qautitative PCR detection of the cultures at d30.
Lin et al. BMC Cell Biology 2012 13:9 doi:10.1186/1471-2121-13-9