Abnormal centrosome amplification and multinucleation. The immunoflorescent microscopy images (A-G) show abnormal centrosome amplification and multinucleation observed in GFP-aurC-WT, GFP-aurC-CA with negative control GFP-alone. (A&B) more than two centrosomes/cell appeared as white dots with anti-γ tubulin staining in GFP-aurC-CA and GFP-aurC-WT respectively. (C) and (D) show multinucleation (more than one nucleus/cell in GFP-aurC-CA and GFP-aurC-WT respectively. (E) Two centrosomes per cell and only one nucleus/cell in G2 phase of GFP-aur-KD. (F) histogram showing the percentage of cells with more than 2 centrosomes/cell of 96 hours after transient transfection in GFP-aurC-CA, GFP-aurC-WT and GFP-alone cells. (G) histogram shows the percentages of multinucleated cells of 96 hours after transient transfection in GFP-aurC-CA, GFP-aurC-WT and GFP-alone.
Khan et al. BMC Cell Biology 2012 13:8 doi:10.1186/1471-2121-13-8