Palmitoylation stabilizes homodimerization. (a) FRET analysis was performed after transfecting combinations of CFPOPRM1/CFPC170A and YFPOPRM1/YFPC170A into HEK cells. (b-c) FLAGOPRM1/FLAGC170A and HAOPRM1/HAC170A were transfected into HEK cells. The colocalization between FLAG-tagged receptor and HA-tagged receptor was determined in (b). The amounts of HA-tagged receptor precipitated with Flag-tagged receptor were normalized against the amount of HAOPRM1 precipitated with FLAGOPRM1 and summarized in (c). One-way ANOVA with Dunnett's test as a post-hoc test was used for analysis. The error bars and "*" represent the standard deviations and significant changes (p < 0.05, n > 3), respectively.
Zheng et al. BMC Cell Biology 2012 13:6 doi:10.1186/1471-2121-13-6