SNF8 binding to TRPC6 in the two-hybrid assay. A. Yeast carrying the pGBKT7 plasmid encoding for the Gal4 DNA binding domain fused to the indicated TRPC6 amino acids, and either pGADT7 (encoding for the GAL4 activation domain alone) or pGADT7-SNF8 (expressing full-length SNF8 fused to the GAL4 activation domain) were grown on high stringency selective media, SD-ade, his, leu, trp (left panel), to assess for protein-protein interaction, or on SD-leu, trp (right panel) as a control. B. Schematic of TRPC6 structure indicating the location of various structural domains relative to the TRPC6 constructs used in the yeast-two hybrid assay.
Carrasquillo et al. BMC Cell Biology 2012 13:33 doi:10.1186/1471-2121-13-33