Table 2

Relative amounts of monomeric and dimeric forms in wild-type TC14s and their mutant proteins.*

Monomer (%)

Dimer (%)


TC14-3, wild

< 1.0

> 99.0


TC14-2, wild

38.9

61.1


TC14-3F65D

100

0


TC14-3T69R

33.3

64.2


TC14-2R69T**

15.7

69.3


*, SDS-PAGE was done under non-heated condition. After staining, each band was scanned with a gel scanner.

**, TC14-2R69T had a few intermediate bands, so that the sum of monomer and dimer did not attain to 100%.

Kawamura et al. BMC Cell Biology 2012 13:3   doi:10.1186/1471-2121-13-3

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