Additional file 3.
Experimental procedure for chimeric protein production. Both cDNA of TC14-2 and TC14-3 were mutated at the position Ile61 to make an unique site for Hind III (top). They were cut with Hind III to exchange the C-terminal fragments with each other (middle). After ligation, chimeric cDNAs were mutated again to change Phe61 to Ile61 before transferred to expression vectors (bottom).
Format: JPEG Size: 226KB Download file
Kawamura et al. BMC Cell Biology 2012 13:3 doi:10.1186/1471-2121-13-3