Additional file 1.

Expression of PmEed during asexual life span in P. misakiensis. (A)RT-PCR of Pmβ-actin (lanes 1-3) and PmEed (lanes 4-6). Lanes 1,4, Growing bud. Lanes 2,5, Juvenile (2- or 3-week-old) zooid. Lanes 3,6, Adult zooid. (B-F)In situ hybridization of PmEed. (B)Growing bud, distal tip. Bar, 50 μm. (C, D)Juvenile zooid. (C)Pharynx. Bar, 50 μm. (D)Ventral body wall. Bar, 25 μm. (E, F)Adult zooid. (E)Intestine and surrounding perivisceral epithelium. Bar, 50 μm. (F)Gonad. Bar, 50 μm. c, coelomic cell; e, epidermis; i, intestine; o, oocyte; p, pharynx.

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Kawamura et al. BMC Cell Biology 2012 13:3   doi:10.1186/1471-2121-13-3