Calcium-binding kinetics of wild-type and mutant TC14s. (A)Wild type TC14-2 and TC14-3. Note that the Ca2+-binding affinity of TC14-3 is lower than that of TC14-2. (B)TC14-3E106G, TC14-3N109G, and TC14-3K113S.N114E. TC14-3N109G showed lower Ca2+-binding affinity than wild-type TC14-3 and TC14-3K113S.N114G showed the higher affinity to some extent.
Kawamura et al. BMC Cell Biology 2012 13:3 doi:10.1186/1471-2121-13-3