Open Access Research article

The adhesion modulation protein, AmpA localizes to an endocytic compartment and influences substrate adhesion, actin polymerization and endocytosis in vegetative Dictyostelium cells

Elizabeth F Noratel, Chere’ L Petty, Jessica S Kelsey, Hoa N Cost, Nisha Basappa and Daphne D Blumberg*

Author Affiliations

Department of Biological Sciences, University of Maryland, Baltimore County 1000 Hilltop Circle, Baltimore, MD, 21250, USA

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BMC Cell Biology 2012, 13:29  doi:10.1186/1471-2121-13-29

Published: 5 November 2012

Additional files

Additional file 1:

Phagocytosis is not significantly altered inampAmutants. Supplemental figure and legend.

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Additional file 2:

Quick Time Movie Wt cells migrating on top of agar to folic acid −2 frames per second.

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Additional file 3:

Quick Time Movie KO cells migrating on top of agar to folic acid-2 frames per second.

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Additional file 4:

Quick Time Movie OE cells migrating on top of agar to folic acid-2 frames per second.

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Additional file 5:

Amp A influences the level of F-actin in growing Dictyostelium cells. Supplemental figure and legend.

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Additional file 6:

AmpA overexpressing cells cannot penetrate a thick bacterial lawn.

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Additional file 7:

The area of the cell in contact with the substrate is influenced by AmpA in an environment dependent manner.

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Additional file 8:

Quick Time Movie Wt cells migrating under agar to folic acid-2 frames per second.

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Additional file 9:

Quick Time Movie KO cells migrating under agar to folic acid-2 frames per second.

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Additional file 10:

Quick Time Movie OE cells migrating under agar to folic acid-2 frames per second.

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Additional file 11:

An AmpA-Tap tag fusion protein vector introduced into cells as a linear KpnI-Not I DNA fragment expresses the AmpA-tap tag fusion protein and retains a wild type phenotype while the same plasmid introduced as a covalently closed circular bacterial plasmid shows an AmpA over expressing phenotype. Supplemental figure and legend.

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Additional file 12:

A mRFP-AmpA fusion protein plasmid introduced as an extrachromosomal plasmid correctly expresses the mRFP-AmpA fusion protein and has an overexpressor phenotype. Supplemental figure and legend.

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Additional file 13:

AmpA Tap tag fusion proteins show the same distribution of AmpA protein in vesicles throughout the cells and in a perinuclear compartment as the mRFP-AmpA tagged construct. AmpA-tap tagged OE and AmpA-tap tagged Wt strains show a similar distribution of AmpA protein within the cell. Supplemental figure and legend.

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Additional file 14:

Overexpression of ampA causes multiple endocytic cups to form repeatedly at the same site. Supplemental figure and legend.

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Additional file 15:

Quick Time Movie; Wt cells carrying the ABD-GFP plasmid (Bsr) undergoing endocytosis. Movie-2 frames per second.

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Additional file 16:

Quick Time Movie; AmpA overexpressing cells carrying the ABD-GFP plasmid (Bsr) undergoing endocytosis. Movie-2 frames per second.

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Additional file 17:

Quick Time Movie; AmpA overexpressing cells carrying the ABD-GFP plasmid (Bsr) undergoing endocytosis. Movie-2 frames per second.

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Additional file 18:

Endosomes acidify properly in ampA mutants. Supplemental figure and legend.

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Additional file 19:

There is no defect in the formation of contractile vacuoles in ampA mutants. Supplemental figure and legend.

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