Stress and Loss of Dys and/or Dg alters miRNA expression levels. a) A heatmap of microarray screen data shows that several miRNAs are misregulated when animals are missing Dys and/or Dg. High temperature stress can also induce a change in miRNA expression levels. Genotypes are written across the top where the bottom genotype is the normalization factor for each column. Black indicates no change, yellow upregulation and blue downregulation of the miRNAs listed in the column to the right. Only miRNAs that had a signal above the threshold under at least one of the tested conditions were mapped. miRNAs are listed in the order of increasing levels measured in Oregon R animals where miRNAs below the red arrow were found to be expressed in Oregon R adult males at 25°C. miRNAs altered in each comparison can be grouped into being independent of stress but dependent on genotype, dependent on stress and genotype, or dependent upon stress regardless of the genotype. Misregulated miRNAs fitting into these categories are represented in the bottom portion of the figure in the Venn diagram.
Marrone et al. BMC Cell Biology 2012 13:26 doi:10.1186/1471-2121-13-26