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Open Access Highly Accessed Research article

Identification of novel mitosis regulators through data mining with human centromere/kinetochore proteins as group queries

Aaron R Tipton1, Kexi Wang1, Peter Oladimeji1, Shermeen Sufi1, Zhidong Gu12 and Song-Tao Liu1*

Author Affiliations

1 Department of Biological Sciences, University of Toledo, Toledo, OH, 43606, USA

2 Ruijin Hospital, Shanghai, 200025, China

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BMC Cell Biology 2012, 13:15  doi:10.1186/1471-2121-13-15

Published: 19 June 2012

Additional files

Additional file 1:

Table S1. Compilation of human centromere-kinetochore proteins.

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Additional file 2 :

Table S2. Comparison of the centromere/kinetochore list compiled in this work with GO annotated centromere/kinetochore genes.

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Additional file 3:

Table S3. Summary table of all “top 50” genes that co-express with the 64 core centromere/kinetochore queries in Gene Sorter search.

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Additional file 4:

Table S4. GO enrichment analysis of genes listed in Table.2.

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Additional file 5:

Table S5. Top-ranking co-expressing genes retrieved by CoExSearch program.

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Additional file 6:

: Table S6. Genes co-expressing with the 64 core centromere/kinetochore components in both Gene Sorter and CoExSearch searches. Highlighed genes were experimentally tested in this work.

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Additional file 7:

Table S7. Top-ranking genes whose encoded proteins interact with the core centromere/kinetochore components.

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Additional file 8:

Figure S1. Western blot of GFP-fusion proteins experimentally tested in this work. Except that C4orf46 was fused with a C-terminal GFP tag, all other constructs contain N-terminal GFP. Asynchronous HEK293 (for TRIP13 and KIAA) and HeLa cells (for the rest) were transfected and cell lysates were harvested 24 ~ 48 hrs later for anti-GFP Western blot.

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Additional file 9:

Figure S2. Co-localization of GFP-KIAA1377 with γ-tubulin throughout the cell cycle. HeLa cells transfected with GFP-KIAA1377 were fixed and stained with DAPI (blue) and anti-γ-tubulin antibody (red). In the bottom row, note no bleedthrough of strong γ-tubulin signals to the green channel in the untransfected cell on the left. Bar = 10 μm.

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Additional file 10:

Figure S3. Comparison of subcellular localization of GFP, GFP-CDKN3, GFP-PBK and C4orf46-GFP. Cells undergoing cytokinesis or in interphase or mitosis were probed. DNA is counterstained with DAPI (blue) and microtubules are stained with anti-α-tubulin antibody (red). Note microtubule staining is not always easily discernible because single focal plane images were shown, and the contrast is optimized to show the microtubule bundles at the midbody in cells undergoing cytokinesis. Bar = 10 μm.

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