Table 1

Strains used in this study
Strain Genotype Source
YJR12,YJR24_1 (wild type, wt) MAT α trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhR Lab. Strain
JK9-3da (wt’) MAT a leu2-3,112 trp ura3 rmel his4 HMLa M. Hall
YJR13 (myo1Δ) MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhRmyo1Δ::HIS5+ Lab. Strain
YFR22 (fks1Δ) MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyhRfks1Δ::kanMX4 F. Rivera
YFR23 (chs2Δ) MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyhRchs2Δ::kanMX4 F. Rivera
YJR066W (tor1Δ) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4 B. Rymond
YGP1 (myo1Δtor1Δ) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4, myo1Δ::HIS5+ This study
YOR008C (wsc1Δ) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4 B. Rymond
YES1 (myo1Δwsc1Δ) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4, myo1Δ::HIS5+ E. Santiago
MH346-1a/pJK3-3 JK9-3da ade2 tor2::ADE2/pSEY18::TOR2 M. Hall
SH121 (tor2Δ ptor2ts) JK9-3da ade2 tor2::ADE2/YCplac111::tor2-21ts M. Hall
YGP5 (myo1Δtor2Δptor2ts) JK9-3da ade2 tor2::ADE2/YCplac111::tor2-21tsmyo1Δ::HIS5+ This study
YJR12/pEJ23 (wt pHA-NPR1) MAT α trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhR, pHA-NPR1 This study
YJR13pEJ23 (myo1Δ pHA-NPR1) MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhR, myo1Δ::HIS5 + pHA-NPR1 This study
(YFR22pEJ23) (fks1Δ pHA-NPR1) MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyhRfks1Δ::kanMX4, pHA-NPR1 This study
YFR23pEJ23 (chs2ΔpHA-NPR1) MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyhRchs2Δ::kanMX4, pHA-NPR1 This study
YJR066WpEJ23 (tor1ΔpHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4, pHA-NPR1 This study
YGP1pEJ23 (myo1Δtor1Δ pHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4 myo1Δ::HIS5+, pHA-NPR1 This study
YDL047W pEJ23 (sit4ΔpHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 sit4Δ::kanMX4, pHA-NPR1 This study
YGP3pEJ23 (myo1Δsit4Δ pHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 sit4Δ::kanMX4 myo1Δ::HIS5+, pHA-NPR1 This study
YOR008C pEJ23 (wsc1ΔpHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4, pHA-NPR1 This study
YES1pEJ23 (myo1Δwsc1Δ pHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4 myo1Δ::HIS5+, pHA-NPR1 This study
YJR24_1/YCplac111 (wt ptor2ts) MAT α trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhR, YCplac111::tor2-21ts This study
YJR13/YCplac111 (myo1Δ ptor2ts) MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhRmyo1Δ::HIS5+, YCplac111::tor2-21ts This study
YFR23/pYCplac111 (chs2Δptor2ts) MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyhRchs2Δ::kanMX4, YCplac111::tor2-21ts This study
YJR13YCplac111pRS316MYO1 (myo1Δptor2tspMYO1) MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhRmyo1Δ::HIS5+, YCplac111::tor2-21ts pRS316MYO1 This study
YGP5pRS316MYO1 (myo1Δtor2Δptor2tspMYO1) JK9-3da ade2 tor2::ADE2, myo1Δ::HIS5+, YCplac111::tor2-21ts pRS316MYO1 This study

Pagán-Mercado et al.

Pagán-Mercado et al. BMC Cell Biology 2012 13:13   doi:10.1186/1471-2121-13-13

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