MS-275 reduced the expression of invasive genes including ROCK1 in HD matrices. The HDAC inhibitor, MS-275, was used to treat cells 24 h after seeding in HD matrix and the cells were harvested 48 h after treatment for either qPCR or for ROCK activity assay. A, Real-time PCR results showed a significant decrease in the expression of TGF-β1, fascin and ROCK1 following treatment with MS-275. Relative expression was calculated using ΔΔCt method. B, Western blotting and activity assay for ROCK1 demonstrated, the reduction in protein level and its activity level following treatment of MS-275. Ratio measurements were obtained following normalisation with α–tubulin. Bars indicate standard error from two biological replicates experiments and experiments were repeated three times. 1-way ANOVA followed by post-hoc Tukey’s test indicating significant difference at ** p < 0.01.
Raviraj et al. BMC Cell Biology 2012 13:12 doi:10.1186/1471-2121-13-12