Additional file 1.
Video 1 This video shows a transgenic C. elegans larva expressing Q82::GFP fusion protein. Several aggregates are formed during the course of filming. One hour of real time elapses in this video, and each frame represents 1 minute of real time. Brightness and contrast of the green-pseudocolored GFP fluorescence channel have been increased from the original images to show the diffuse, soluble material. A single DIC image is repeated for every frame and serves as an anatomical reference. The brightness and contrast have been adjusted for the DIC image to enhance visualization of the GFP channel. A 10X lens with a 2X optvar was used, to give 200X total magnification. Video plays at 10 frames/sec with each frame representing one minute of recording time.
Format: MOV Size: 231KB Download file
Skibinski and Boyd BMC Cell Biology 2012 13:10 doi:10.1186/1471-2121-13-10