Figure 4.

Q82::GFP aggregates contain both K48- and K63-linked polyubiquitin chains. Synchronized adult worms were fixed and probed with antibodies specific for K48 or K63 polyubiquitin chains. Secondary antibody was conjugated to TRITC (A, D, and G). Aggregates were visualized via GFP fluorescence (B, E, H). Control samples (G) received the same treatment, without primary antibody. Merged images (C, F) show colocalization of K48- or K63-linked polyubiquitin chains to aggregates, but not when the primary antibody is omitted (I).