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Open Access Research article

Ubiquitination is involved in secondary growth, not initial formation of polyglutamine protein aggregates in C. elegans

Gregory A Skibinski and Lynn Boyd*

Author Affiliations

Department of Biological Sciences, University of Alabama in Huntsville, Huntsville AL 35899, USA

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BMC Cell Biology 2012, 13:10  doi:10.1186/1471-2121-13-10

Published: 11 April 2012

Additional files

Additional file 1:

Video 1 This video shows a transgenic C. elegans larva expressing Q82::GFP fusion protein. Several aggregates are formed during the course of filming. One hour of real time elapses in this video, and each frame represents 1 minute of real time. Brightness and contrast of the green-pseudocolored GFP fluorescence channel have been increased from the original images to show the diffuse, soluble material. A single DIC image is repeated for every frame and serves as an anatomical reference. The brightness and contrast have been adjusted for the DIC image to enhance visualization of the GFP channel. A 10X lens with a 2X optvar was used, to give 200X total magnification. Video plays at 10 frames/sec with each frame representing one minute of recording time.

Format: MOV Size: 231KB Download file

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Open Data

Additional file 2:

Video 2 This video shows aggregation prone fluorescent Q82::GFP fusion protein expressed in the body wall muscle cells of a transgenic C. elegans larva. An aggregate is seen forming in the lower right of the image. The video represents 40 minutes of real time. Video plays at 10 frames/sec, with each frame representing one minute of recording time. A 10X lens with a 2X optvar was used, to give 200X total magnification. Brightness and contrast were increased to better show the diffuse, soluble material. Scale bar indicates a distance of 10 microns.

Format: MOV Size: 39KB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data