Expression and localization of transgelin family proteins in REF52 cells. A, western blot analysis of SM22, NP25 and NP22 levels in various cell lines as indicated (S3T3 = Swiss 3T3). REF52 cells were chosen for further analysis due to their amenable phenotype and the expression of only SM22α. B-G, REF52 cells stained with Alexa 488 phalloidin (red in merge) to reveal F-actin containing structures (B, E), and stained for endogenous SM22α (C), or expressing SM22α-GFP (F) green in merges. Merged images (D, G) all show strong co-localization between endogenous and exogenous SM22 proteins and F-actin stress fibres. Nucleus counterstained with DAPI (blue).
Thompson et al. BMC Cell Biology 2012 13:1 doi:10.1186/1471-2121-13-1