Table 1

Auxin-induced growth defects in aid-tagged strains

Gene

Protein function

AID

i-AID

off-AID


mcm4

MCM subunit

+*

++*

NT

orc2

ORC subunit

NT

++*

NT

orc4

ORC subunit

NT

++*

++*

orc6

ORC subunit

NT

+*

NT

psf1

GINS subunit

NT

+*

++*

pol1

catalytic subunit of Polα

NT

-*

++*

pol12

second largest subunit of Polα

NT

+*

++*

spp2

primase subunit of Polα

NT

+*

++*

cdc20

catalytic subunit of Polε

NT

-*

++*

dpb2

second largest subunit of Polε

NT

+*

++*

cdc45

replication factor

NT

+*

++*

mcm10

replication factor

NT

-

++*

hsk1

catalytic subunit of DDK

NT

-

++

ssl3

cohesin loader

NT

-

++

cia1

histone chaperone

NT

-

++


++: severe growth defect on plates containing 0.5 mM auxin for AID and i-AID, or 0.5 mM auxin with 10 μg/ml thiamine for off-AID, +: slow growth on plates containing 0.5 mM auxin, -: no effect, NT: not tested, *: defect of DNA replication observed in flow cytometry analysis

Kanke et al. BMC Cell Biology 2011 12:8   doi:10.1186/1471-2121-12-8

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